Establishment of 9 LBCL PDX models. (A) Hematoxylin and eosin stains of 9 LBCL PDX models propagated under the renal capsule of NSG mice. Original magnification ×200. (B) Epstein-Barr-encoded RNA in situ hybridization of all 9 LBCL PDX models. An EBV-positive classical Hodgkin lymphoma (cHL) served as positive control. Bars represent 100 μm. (C) Clonality of the 9 PDX models assessed and interpreted according to the EuroClonality/BIOMED-2 guidelines.⁴⁵  An IgH V_H-J_H spanning PCR (FR2, left) was performed on all 9 LBCL PDX models. Tumors without a clonal peak (LTL-005, LTL-037, and LTL-048) or an inconclusive result (LTL-037) were sequentially analyzed with a second PCR (FR1, right). Genomic DNA (gDNA) of NSG mouse tail served as a negative control, gDNA of peripheral blood mononuclear cells from a healthy human volunteer served as a polyclonal control, and gDNA of the Burkitt lymphoma cell line (BL30) served as a monoclonal control. EBV, Epstein-Barr virus; FR, fragment; PCR, polymerase chain reaction.