Molecular heterogeneity of LBCL PDX models. (A) Heatmap of the relative expression of ABC- and GCB-signature genes³⁷  in ABC- and GCB-type DLBCL PDX models and the additional PBL PDX model. Note that reads aligned to the mouse genome were filtered before the DLBCL PDX models were classified by COO.³  (B) Gene set enrichment analyses of the ABC-signature genes (upper) and GCB-signature genes (lower) in the ABC- and GCB-type DLBCL PDX models. (C) Detected IGH-MYC (upper) and IGH-BCL2 (lower) translocations in LTL-014 and LTL-030, respectively. Translocations are plotted in their genomic context. Exons are visualized as boxes, with ATG-containing exons in red, coding regions underlined in green, and enhancer regions underlined in black. Numbers of supporting reads (split reads, read pairs) are indicated above, and individual supporting reads are shown below. (D) Protein-perturbing mutations with an allele fraction >0.1 in the LBCL models. Mutations in most frequently reported recurrently mutated genes in primary DLBCL (supplemental Table 2C) are visualized as a color-coded heatmap (red, missense mutation; green, frameshift mutation; purple, nonsense mutation; brown, splice site mutation; white, mutation absent); CNAs in TP53 and CDKN2A are represented as a color-coded heatmap (dark blue, biallelic loss; light blue, monoallelic loss; white, no loss). COO transcriptional subtypes and identified translocations juxtaposing either BCL2 or MYC to the IgH locus are indicated in the legend above of the heatmap. ES, enrichment score.