Analyses of cell surface immunoglobulin and BCR signaling in the LBCL PDX models. (A) Single-cell suspensions from each LBCL PDX model were gated for human CD45-positive cells and analyzed for surface immunoglobulin (IgG, red; IgM, orange; isotype, gray). CCC and COO subtypes of each model are indicated above the flow histograms. (B) Cellular proliferation of PDX tumor cell suspensions after chemical SYK inhibition with entospletinib (GS-9973) for 24 hours. (C,D) HRK (C) and BCL2A1 (D) transcript abundance following entospletinib (GS-9973) treatment. Experiments were performed in triplicate. A representative experiment of biological duplicates in shown. DMSO, dimethylsulfoxide. The P values were obtained using a Student t test (*P < .05).