Plot showing combined reactivities of Q237, Q328, and Q366 in fibrinogen αC (233-425). (A) A combined graph showing the rate of consumption of all three reactive glutamines Q237 (♦), Q328 (▪), and Q366 (▲). The peak-height ratio method was used to calculate the amount of reactant left in triplicate experiments and plotted as mean ± standard deviation. These reactive glutamines were ranked as Q237 ≫ Q366 ≈ Q328. (B) An increase in FXIIIa concentration by threefold shows that Q366 reacts comparably with Q328 even at higher time points.