Delayed rhADAMTS13 administration 60 minutes after occlusion is able to restore MCA blood flow and reduce ischemic brain injury. A large thrombus was generated in the right MCA of C57Bl/6J mice. Sixty minutes after inducing a stable MCA occlusion, mice were treated with either rhADAMTS13 (3500 U/kg) or vehicle (arrow). MCA blood flow was monitored via laser Doppler flowmetry to assess recanalization of the MCA until 1 hour after injection. (A) In mice treated with rhADAMTS13, a significant increase in blood flow was observed. (B) Infarct sizes were significantly decreased in mice that received rhADAMTS13. Quantification of the infarct sizes is shown in the left panel, and representative brain sections are shown in the right panel. (n = 8 mice for each group; *P < .05; ****P < .001 compared with vehicle.)