Figure 2
Figure 2. Potency assay gating. (A) Debris-free gate. (B) Gating of the nonviable (7-AAD+) and RBC (PE-Cy5-GlyA+) cells. (C) Gating of the PE-CD45+ cells. (D) Gating of APC-CD34+ cells. (E) Gating of ALDHbr cells. (F) Gating of cells stained with the Aldecount reagent in the presence of the inhibitor DEAB. BAA, BODIPY aminoacetate; FSC, forward scatter; SSC, side scatter.

Potency assay gating. (A) Debris-free gate. (B) Gating of the nonviable (7-AAD+) and RBC (PE-Cy5-GlyA+) cells. (C) Gating of the PE-CD45+ cells. (D) Gating of APC-CD34+ cells. (E) Gating of ALDHbr cells. (F) Gating of cells stained with the Aldecount reagent in the presence of the inhibitor DEAB. BAA, BODIPY aminoacetate; FSC, forward scatter; SSC, side scatter.

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