CL was procoagulant and microparticle-bound. (A) A structural comparison between CL and PS. (B) The time to clot of phospholipid-depleted plasma in the presence of purified CL, PS, or PC (n = 6; 1-way ANOVA, *P < .01 vs PC). (C) Clotting times of plasma collected at different points from C57BL/6J mice subjected to FPI or sham surgery (n = 6; 1-way ANOVA, *P < .01 vs sham mice). (D) Clotting time of plasma collected from non-injured C57CL/6J mice 30 minutes after CL injection (n = 6/group; 1-way ANOVA). (E) hematoxylin and eosin (HE, top) and phosphotungstic acid-haematoxylin (PTAH, bottom) stains of the lungs show perivascular accumulation of erythrocytes resulted from vascular leakage (top left) and intravascular fibrin deposition (arrow, bottom left) in mice injected with CL, but not those injected with PBS (right panels, 5-10 evaluations/treatment). (F) Anti-CL antibody detected CL in microparticles, not microparticle-free plasma (MPFP) collected from mice 6 hours after FPI (MPFP was prepared as previously described¹² ).