Figure 2
![Figure 2. CD40LG gene replacement with a functional CD40LG transgene. (A) CD40LG cDNA gene replacement templates targeting the 5′UTR region of exon 1 of CD40LG: CD40LG[GFP.CD40L.3′UTR] (top) and CD40LG[GFP.CD40L.WPRE] (bottom). Total AAV packaging sizes are 4616 and 4234 bp, respectively. (B) Average HDR rate (percentage GFP+; n = 4 separate healthy male donors) of each gene-editing template in CD4+ T cells. (C) GFP (x-axis) and CD40L (y-axis) coexpression or (D) CD40-muIg binding in T cells edited with cDNA gene replacement templates. (Top row) Resting; (bottom) P/I activated. *Plots in panels C and D were generated at separate times, using different donor T cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/127/21/10.1182_blood-2015-11-683235/4/2513f2.jpeg?Expires=1735844396&Signature=f3Fh6ORI1CDalRMyZrNafqAdIx6nIIkAnp2lT~9pGjaUEnE41uo-T63liW~56Dila-fY6-YECaBOyT5e14uMfVZ4p3b9QM84LsNkoa40u1XmoleNHsEUJTtEtGroxs5D3vsA6hiOl2~3AKfG30hX3Qxkg8xuWEbwWxftXoxcWO~r8qdADdLLJNC0rQDj93q2QJko71-TVbIcF45Kv38hxkqCjHJOeHOygKTB2pzhMmJQhvvzTc7Bf9vZPz6yDjF-HWyEe9SS6IkmqS1JK0OQ2AAodnuFarNk74T~LzZNLzFvQvxvIjXgFxMAGmNqUzlfJUtaKwBnIeGG8jvhxURDvA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
CD40LG gene replacement with a functional CD40LG transgene. (A) CD40LG cDNA gene replacement templates targeting the 5′UTR region of exon 1 of CD40LG: CD40LG[GFP.CD40L.3′UTR] (top) and CD40LG[GFP.CD40L.WPRE] (bottom). Total AAV packaging sizes are 4616 and 4234 bp, respectively. (B) Average HDR rate (percentage GFP+; n = 4 separate healthy male donors) of each gene-editing template in CD4+ T cells. (C) GFP (x-axis) and CD40L (y-axis) coexpression or (D) CD40-muIg binding in T cells edited with cDNA gene replacement templates. (Top row) Resting; (bottom) P/I activated. *Plots in panels C and D were generated at separate times, using different donor T cells.
