Kinetics of CD40L expression in edited X-HIGM T cells are comparable to that of healthy donor T cells. (A) Representative time course of CD40L surface expression (as MFI histogram overlay) on healthy control CD4⁺ T cells (top) and X-HIGM CD4⁺ T cells edited with CD40LG[GFP.CD40L.3′UTR] (bottom). Histograms were collected 0, 0.5, 2, 3, 8, 24, and 54 hours following P/I activation (0). P/I was removed (washed off) at the 3-hour time point. Average MFI (B) and percentage (C) of CD40L surface expression on T cells over time. The shaded area shows the nonedited healthy control expression pattern. Error bars in panels B and C indicate the range of values, not SD, in order to show donor-to-donor variability.