UV-HSV-1 potently induces activation of NK cells. (A) PBMCs from 6 healthy donors were exposed to UV-HSV-1 (0.1 pfu/PBMC for 16 hours), and expression of CD69 was determined by flow cytometry as described in “Materials and methods.” (B-C) PBMCs from 2 healthy donors were exposed to increasing concentrations of UV-HSV-1 for 16 hours, and CD69 expression was determined by flow cytometry. *P < .001 from unexposed PBMCs. (D) PBMCs from 3 healthy donors were exposed to UV-HSV-1 (0.1 pfu/PBMC for 16 hours) and degranulation (CD107a externalization) was determined by flow cytometry as described in “Materials and methods.” (E-F) PBMCs from 6 healthy donors were exposed to UV-HSV-1 (0.1 pfu/PBMC for 16 hours), and the % of cells positive for IFN-γ and the relative amounts of IFN-γ in positive cells were determined by flow cytometry as described in “Materials and methods.” (G) PBMCs from 3 healthy donors were exposed to UV-HSV-1 (0.1 pfu/PBMC for 16 hours), and migration toward OCI-AML3 conditioned media was determined by flow cytometry as described in “Materials and methods.” (H-J) PBMCs from 2 healthy donors were exposed to increasing concentrations of UV-HSV-1 for 16 hours, and CD107a externalization and % of IFN-γ⁺ cells was determined by flow cytometry. *P < .05 from unexposed controls.