MF CD34⁺ cells are more sensitive to the effects of LCP4. (A-E) Splenic MF and normal BM CD34⁺ cells were cultured in the presence of cytokines (50 ng/mL SCF + 100 ng/mL TPO) alone or cytokines plus LCP4 at doses ranging from 10 nM to 500 nM for 1 week. After treatment, cells were analyzed by mAb staining and flow cytometry. A proportion of cells generated in these cultures were also assayed in semi-solid media to assess the effect of LCP4 on MF and normal HPCs. The percentage of the total number of CD34⁺Lin⁻ cells (A), CFU-MK (B), CFU-GM (C), as well as mature MKs (CD41a⁺CD34⁻CD15⁻) (D) and myeloid cells (CD15⁺CD34⁻CD41a⁻) (E), generated in the cultures exposed to cytokines plus LCP4 relative to those generated in the cultures exposed to cytokines alone is shown. *P < .05; **P < .01; ***P < .001; ^#P = .06; each dose of LCP4 vs 0 nM of LCP4 unless specified in the graphs. Splenic MF and N BM: n = 7 each. N BM, normal BM.