The switch in deformability in mature stages occurs upon STEVOR S₃₂₄ dephosphorylation. (A) Immunoprecipitation of stage III and stage V GIE lysates from the Full-Ty1 line with anti-Ty1, anti-GST, and anti-phospho-PKA antibodies. Immunoblots were probed with a rabbit antibody directed against STEVOR (PFC0025c). (B) Retention rates in microsphilters of stage V GIE from the control (light gray), the Full-Ty1 (medium gray), and the Full_S324A-Ty1 (dark gray) lines. (C) Retention rates in microsphilters of stage V GIE from the control (light gray) and the Full_S324A-Ty1 (dark gray) lines preincubated at 37°C 30 minutes with (+cAMP) or without (−cAMP) 100 µM 8Br-cAMP. (D) Retention rates in microsphilters of stage V GIE from the control (light gray), the Null (medium gray), and the Full_S324A-Ty1 (dark gray) lines preincubated at 37°C 30 minutes with (+Sildenafil) or without (Untreated) 100 µM sildenafil citrate. Error bars denote the standard error of the mean. Outliers are shown as open circles. Stars represent significant differences in retention rates (***P < .001; **P < .01; *P < .05). ns, nonsignificant differences in retention rates.