Surface phenotype of TCM-enriched CD19R+ cells. Freshly thawed T-cell products of (A) patient NHL1-4 and (B) patient NHL 2-2 were examined for the expression of CD3, CD4, CD8, CD62L, CD28, CD27, CD127, and PD-1 by flow cytometry. Percentages of positive cells (filled histograms) are calculated using the subtraction method on the basis of the gating of isotype-stained cells (open histograms), except CD4 and CD8 using indicated gating strategy. (C) Percentages of positive cells from all the patients are depicted. NHL1 patients are represented by filled circles and NHL2 patients by open circles. Means are presented for each surface phenotype.