(A) Logarithmic scale of the absolute number of nucleated cells after CD25⁺ immunomagnetic separation and at the end of the KT64/86-stimulated culture, of which the median proportion of CD4⁺CD25⁺ cells was 97% (range, 88-99) and with 87% (range, 78-95) expressing CD4⁺FoxP3⁺CD127^–. (B) The median proportion, interquartile range, and range of CD4⁺FoxP3⁺CD127^lowHelios⁺ cells that expressed the surface markers CD62L, OX40, and 41BB. (C) The median proportion, interquartile range, and range of chemokine receptors expression on CD4⁺FoxP3⁺CD127^lowHelios⁺ cells; CXCR3⁺ (Th1-like), CXCR3^–CCR6^– (Th2-like), CXCR3^–CCR4⁺CCR6⁺CCR10^– (Th17-like), and CXCR3^–CCR4⁺CCR6⁺CCR10⁺ (Th22-like). (D) The median proportion, interquartile range, and range of CD4⁺FoxP3⁺Helios⁺ cells that after ionophore and PMA stimulation expressed IFNγ, TNFα, IL-2, IL-4, IL-10, and IL-17. (E) The median proportion, interquartile range, and range of utilization of 24 v beta-chains in CD4⁺CD25⁺⁺ cells from the Treg product. (F) The proportion of CD4⁺FoxP3⁺CD127^low cells derived from the expansion product detected in the PB of the patients with an informative HLA marker (n = 7). The black lines on all panels represent the median values.