Heterogeneity of the phenotypic myeloid progenitor pool in NHD13 mice. Bone marrow (BM) was harvested from 25-week-old male and female NHD13 (n = 5) and WT (n = 4) littermates via the crushing technique. BM was analyzed for progenitor frequencies via flow cytometry. (A) Representative flow cytometric gating schema on the whole BM from a 25-week-old WT mouse in which common lymphoid progenitors (CLPs) (Lin⁻/Flt3⁺/IL7R⁺) and myeloid progenitors (MPs) (Lin⁻/c-Kit⁺/Sca1⁻) are a subset of the lineage-negative (live) cell population, the latter of which can be divided into common myeloid progenitors (CMPs) (FcγR⁻/CD34⁺), megakaryocyte-erythrocyte progenitors (MEPs) (FcγR⁻/CD34⁻), and granulocyte-macrophage progenitors (GMPs) (FcγR⁺/CD34⁺). (B-D) Quantification of progenitor populations in WT compared with NHD13 littermates. In all graphs, each dot represents an individual mouse, and color denotes the same individual mouse in each gate. *P < .05; ***P < .001.