tfec expression modulates caudal endothelial cytokine expression. (A) Experimental outline. (B-E) qPCR data showing gene expression in FACS-sorted cECs after NI (n = 5 for all), hl-ztfec (+tfec; n = 4 for all, except csf3b, where n = 5) or DN-tfec (+DN; n = 3 for all) injection. Each n represents an average of biological triplicates; experiments were repeated at least 3 times. (F) ISH for cmyb at 4 dpf in WT/tfec^+/− embryos or tfec^−/− mutant embryos after noninjection (NI) and kitlgb (+kitlgb) injection. (G) Analysis of cmyb expression.