Bim downmodulation is associated with prosurvival responses to TLR9 stimulation. (A) Expression profile of BH3 proteins in CLL cells treated with or without DSP30 for 72 hours. Data show western blot analysis of n = 4 representative patients samples out of n = 10 tested. Samples were encrypted and anonymized by V numbers. (B) Percentage of ABT-737–induced apoptosis was calculated in relation to the respective untreated controls. CLL cells were precultured with or without DSP30 for 24 hours and subsequently treated with 5 nM ABT-737 for 24 hours. Viability was assessed by determining the percentages of annexin-V/PI–negative cells. Data are presented as the mean ± SD of n = 18 patient samples in a bar diagram. P values are indicated by asterisks: **P < .01. (C) Expression profile of antiapoptotic proteins in DSP30-treated CLL cells with or without ABT-737 treatment (5 nM) for 24 hours. Data show representative western blot analysis of n = 4 tested patients samples.