Transduced cells persist long-term and proliferate. All values shown are medians; black dotted lines represent minimum and maximum values. The number of patients contributing to each data point is indicated on the graph. Day 0 indicates baseline (pre-GT) values. (A) CD34⁺ cells were purified from bone marrow aspirate. (B-K) CD15⁺ cells (B), CD3⁺ cells (C-D), CD19⁺ cells (E-F), CD16⁺CD56⁺ cells (G-H), CD3⁺CD4⁺ cells (I), CD3⁺CD8⁺ cells (J), and CD4⁺CD45RA⁺ cells (K) were purified from peripheral venous blood. (A-C,E,G) Genotype was determined by qRT-PCR. (D,F,H-K) Absolute cell counts are also shown; 10 was set as the minimum value for graphs using a logarithmic scale. The shaded blue and purple regions represent median and fifth percentile values, respectively, in normal children. The top edges correspond to levels in children ages 2 to 5 years; bottom edges correspond to levels in children ages 10 to 16 years. Values for children ages 5 to 10 typically fall within the shaded areas.²⁶  The green and orange shaded areas represent median and 10th percentile values, respectively, in normal children. Top edges correspond to levels in children ages 2 to 6 years; bottom edges correspond to levels in children ages 12 to 18 years. Values for children ages 6 to 12 years fall within the shaded areas.²⁷