Measurement and functional characterization of HSPCs in Klf5^Δ/Δ mice. (A) Measurement of total HSPC (defined by IL7Rα⁻c-Kit⁺Sca-1⁺), LT-HSCs (defined by c-kit⁺Lin⁻IL7Rα⁻Sca-1⁺CD48⁻CD150⁺), ST-HSCs (defined by c-kit⁺Lin⁻IL7Rα⁻Sca-1⁺CD48⁺CD150⁺), and MPPs (defined by c-kit⁺Lin⁻IL7Rα⁻Sca-1⁺CD48⁺CD150⁻) as a percentage of the cKit⁺Lin⁻ compartment from bone marrow of mice at 9 months of age using flow cytometry. Results were obtained from n = 8 Klf5^fl/fl, n = 8 Klf5^wt/Δ, and n = 11 Klf5^Δ/Δ mice. (B) Total HSPCs, MPPs, and ST-HSCs (as a percentage of the cKit⁺Lin⁻ compartment) in the spleen of 12-month-old mice. Results were obtained from n = 7 Klf5^fl/fl, n = 5 Klf5^wt/Δ, and n = 8 Klf5^Δ/Δ mice. (C) Enumeration of myelo-erythroid colonies from spleen cells of Klf5^Δ/Δ mice at 9 and 12 months old. Results were obtained from n = 6 Klf5^fl/fl, n = 5 Klf5^wt/Δ, and n = 7 Klf5^Δ/Δ mice at 3 months and n = 6 Klf5^fl/fl, n = 4 Klf5^wt/Δ, and n = 6 Klf5^Δ/Δ mice at 9 months. (D) Quantification of myelo-erythroid progenitor self-renewal from bone marrow using serial colony-forming assays at 9 months. Results were obtained from n = 4 Klf5^fl/fl, n = 5 Klf5^wt/Δ, and n = 5 Klf5^Δ/Δ mice. Mean and SEM are shown for all measurements. P values were obtained from 2-tailed t tests conducted between each genotype group: ***P < .001, **P < .01, and *P < .05.