Expression of F8 and vWF mRNA in fluorescence-activated cell sorted subtypes of ECs from various tissues. Lymphoid tissue ECs were released from the surrounding stroma by enzymatic digestion while preserving their viability, and the sorted EC subsets were then transcriptomically profiled. The transcriptomes of the sorted EC subtypes were combined with data from sorted ECs^16,17  analyzed with the same microarray platform (Affymetrix Mouse Gene 1.0 ST arrays) and normalized on the basis of GeneSpring preprocessing and default normalization (RMA-16). The levels of F8 and vWF mRNA were plotted from these normalized transcriptomes. Raw Affymetrix expression values of at least 3 biological replicates (except n = 1 for PLN CAP; some values were excluded because of contamination with HEV-specific genes). Mean ± standard error of the mean. BM, bone marrow; cap, capillary; CNS, central nervous system; FRC, fibroblastic reticular cell; LP, lamina propria; MLN, mesenteric lymph node; PLN, peripheral lymph node; PP, Peyer’s patches SLN, subcutaneous lymph node.