Expression profiles of LSC markers on paired AML diagnosis and relapse samples. (A) A representative example of LSC marker immunophenotyping strategy in AML bulk cells (AML3, diagnosis). The LSC panel included CD32, CD33, CD45RA, CD47, CD96, CD97, CD99, CD123, HLA-DR, IL1RAP, and TIM-3 (top). (B) Frequencies of LSC marker positive populations (CD123⁺ and TIM-3⁺) in bulk AML cells at diagnosis (middle) and relapse (right) compared with NBM cells (left) (NBM, n = 22-23; AML, n = 24-25). Mean ± standard error of the mean values are plotted. *P < .05; **P < .01; ***P < .001; ****P < .0001. NS, not significant.