Analysis of mRNA translation using OPP labeling. (A) Overview of experimental procedure for OPP labeling. (B) CLL samples (n = 13) were treated with soluble anti-IgM or anti-IgM beads, control antibodies, or CpG-ODN2006 or left untreated for 24 hours before OPP labeling. Graphs show fold increase in OPP labeling (means ± SEM) in CLL (CD19⁺CD5⁺) and T cells (CD19^–CD5⁺), with values for untreated CLL cells set to 1.0. Statistical comparisons between untreated CLL and T cells, and between control and anti–IgM-treated CLL cells are shown (Student t test). (C-D) As in (B), except cells were pretreated for 1 hour with ibrutinib (n = 5) or tamatinib (n = 5), or DMSO was used as a control, before the addition of anti-IgM/control antibodies. The graphs show fold increase in OPP labeling (means ± SEM) for T cells (C) and CLL cells (D), with values for untreated cells set to 1.0. Statistical comparisons between groups are shown (Student t test).