LIN28B defines a fetal-like stem cell signature in JMML patients. (A) Embryonic hemoglobin/HbF genes are enriched in patients with high LIN28B levels (gene set enrichment analysis). All genes measured on microarray are ranked from left to right on the basis of their differential expression between LIN28B-high and -low patients. Every vertical black line represents a gene in the hemoglobin gene set, and all other genes are invisible. The graph shows that most genes in the hemoglobin gene set are more highly expressed in patients with high LIN28B expression (more to the left). (B) Log2 expression of HBBP1 in LIN28B-low and -high patients. (C) Log2 expression of HBG1 in LIN28B-low and -high patients. (D) Heat map with expression levels of the 20 most negatively correlating miRNAs with LIN28B. Red indicates high expression; blue indicates low expression. (E) Spearman rank correlation of the top 20 most anticorrelating miRNAs with LIN28B. (F) Fetal hematopoiesis genes are enriched in patients with high LIN28B levels (gene set enrichment analysis). (G) Log2 expression of HMGA2 in LIN28B-low and -high patients. (H) Log2 expression of IGF2BP3 in LIN28B-low and -high patients. FDR, false discovery rate; NES, normalized enrichment score.