RNA sequencing of FLK1 mesoderm targeted by Eng −8/P/LacZ/+7/+9 identifies genes associated with hemangioblast activity. (A) RNA-sequencing profiles shows Kdr (Flk1) transcripts (top) and Eng transcripts (bottom) in the F+/L− and F+/L+ fractions. FPKM expression values are shown to the right. (B) (i) Heat map representation of up- and downregulated genes in FLK1+/LacZ− (F+/L−) and FLK1+/LacZ+ (F+/L+) fractions in 3 independent experiments. (ii) Expression (FPKM values) levels of genes that have previously been associated with hemangioblast function. The left panel shows a subset of genes that are differentially expressed between F+/L− and F+/L+ fractions, and the right panel shows a subset of genes that are not. (C) GSEA profiles shows the correspondence of genes that are differentially expressed between F+/L− and F+/L+ fractions and those that are differentially expressed in ETV2^+/− vs ETV2^−/− (top) and LDB wt vs LDB^−/− gene sets. DEG, differentially expressed genes.