Transcriptomic analysis of HE fractions identifies genes associated with HE to hematopoietic transition. (A) RNA-sequencing profiles show Eng transcripts (top) and Runx1 transcripts (bottom) in the E+/L−, E+/L+, and E−/L+ fractions. FPKM expression values are shown to the right. (B) (i) Heat map representation of up- and downregulated genes in ENG+/LacZ− (E+/L−) HE, ENG+/LacZ+ (E+/L+) HE, and ENG−/LacZ+ (E−/L+) HE fractions in 3 independent experiments. (ii) Expression (FPKM values) levels of genes that have previously been associated with HE. The top panel shows a subset of genes that are differentially expressed between E+/L−, E+/L+, and E−/L+ fractions, and the bottom panel shows a subset of genes that are not. (C) GSEA profiles show the correspondence of genes that are differentially expressed between the E+/L−, E+/L+, and E−/L+ fractions and those that are differentially expressed in EC vs HECs (top) and HECs vs HSC gene sets. (D) TFs and CSRs that are up- and downregulated in the Eng −8/P E−/L+ HE fraction. The log fold changes (logFC) and log false discovery rates (logFDR) are listed for each gene.