Induction of cell death, associated with activated mitochondria and ROS production, is reduced in LICs by Atg7 deletion. Collective data indicating the frequencies of L-GMP cells from vehicle-treated (n = 10) or TAM-treated (n = 11) Atg7^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice (A) and vehicle-treated (n = 6) or TAM-treated (n = 7) Atg5^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice (B). (C) Representative flow cytometric analysis of mitochondrial activity in lineage⁺ and LK cells from vehicle- or TAM-treated Atg7^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice. (D) Collective data of relative signal level of Mitotracker Orange in lineage⁺ and LK cells from vehicle-treated (n = 6) or TAM-treated (n = 7) Atg7^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice. Relative signal represents geometric mean fluorescent intensity (MFI) of each sample divided by the average MFI from all vehicle samples. (E) Representative flow cytometric analysis of ROS levels in lineage⁺ and LK cells from vehicle- or TAM-treated Atg7^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice. (F) Collective data of relative signal levels of CellRox DeepRed in lineage⁺ and LK cells from vehicle-treated (n = 6) or TAM-treated (n = 7) Atg7^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice. (G) Boxplot of the frequencies of annexin-V⁺ or DAPI⁺ cells from vehicle-treated (n = 6) or TAM-treated (n = 7) Atg7^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice. (H) Boxplot of the frequencies of annexin-V⁺ or DAPI⁺ cells from vehicle-treated (n = 6) or TAM-treated (n = 7) Atg5^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice. *P < .05; **P < .01. All results represent pooled data from at least 3 independent experiments with 2 to 4 animals per experiment.