Decreased frequency of functional LICs in Atg7-deleted MLL-ENL mice. (A) Representative flow cytometric plot of donor cell engraftment 16 hours after BMT with 1 × 10⁶ GFP⁺Atg7^flox/flox or Atg7^Δ/Δ cells. (B) Collective data of donor cell engraftment at 16 hours after BMT with 1 × 10⁶ GFP⁺Atg7^flox/flox or Atg7^Δ/Δ cells (n = 7 per group). (C) Kaplan-Meier curves represent survival of tertiary recipients with 3, 30, 300, or 3000 GFP⁺ BM cells from vehicle- or TAM-treated leukemic mice (n = 4-8 per group). (D) Poisson statistical analysis of limiting dilution transplantation assay. Plots were generated for estimation of the frequency of functional LICs in GFP⁺ BM cells from vehicle- or TAM-treated leukemic mice (n = 4-8 at each cell dose). The plot represents the logarithm of the frequency of living mice at 42 days after injection for the indicated number of cells. LIC frequency was 1:46 for vehicle-treated (95% confidence interval, 1:18-115) and 1:301 for TAM-treated (95% confidence interval, 1:126-718) leukemic mice. P = .0002.