Dependence of PB leukemia cells on autophagy. (A) WBC counts of PB from Atg5^flox/flox:Cre-ER^T2 or Atg7^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice were measured at indicated days after BMT (>7 mice per group, mean ± standard deviation [SD]). P values were calculated by a Welch t test. Collective data of frequencies of annexin-V⁺ or DAPI⁺ cells in c-Kit⁻CD11b⁺ or c-Kit⁺CD11b⁺ cells obtained from vehicle- or TAM-treated Atg7^flox/flox:Cre-ER^T2 (B) and Atg5^flox/flox:Cre-ER^T2 (C) mice (n = 6-9 per group). (D) Relative colony numbers from GFP⁺ PB cells sorted from vehicle- or TAM-treated Atg7^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice are shown (n = 4 per group, mean ± SD). (E) Kaplan-Meier curves representing survival of tertiary recipients of GFP⁺ PB cells from vehicle- or TAM-treated Atg7^flox/flox:Cre-ER^T2 MLL-ENL leukemic mice (n = 9 per group). The P value was calculated by a log-rank test. *P < .05; **P < .01. All results represent pooled data from at least 3 independent experiments with 1 to 4 animals per experiment.