Accelerated clearance of VWF N1515Q and VWF N1574Q is mediated by macrophages. (A) In order to assess the potential contribution of macrophages in modulating the enhanced clearance of VWF glycan variants, clearance of VWF N1515Q and VWF N1574Q was repeated in VWF^−/− mice 24 hours after clodronate-induced macrophage depletion. (B) To determine whether macrophages play a role in regulating the reduced survival of A1A2A3-N1515Q and A1A2A3-N1574Q, in vivo clearance studies were also re-assessed in VWF^−/− mice following clodronate treatment. Data are graphed as percentage residual VWF relative to the amount injection (*P < .05, **P < .01, and ***P < .001).