Reduced interaction of VWF with TSP2 KO derived ECM. DFs from WT (A, C) and TSP2 KO mice (B, D) mice were cultured for 10 days and then removed by decellularization. ECMs were then exposed to plasma under flow (15 dynes/cm²) for 15 minutes. (A-B) Immunofluorescence detection of FN (green color) revealed retention of the ECM at the conclusion of the experiment. (C-D) VWF accumulation on ECM was detected by immunofluorescence (green color) (Zeiss). Interaction with TSP2 KO ECM (D) was minimal in comparison with WT (C) and this was confirmed by image analysis using ImageJ (E). n = 3; *P < .05