Ectopic expression of miR-139-5p suppresses the differentiation of LSK and myeloid progenitors. (A) Schematic representation of the MSCV-based retroviral vector with the insertion of miR-139-5p sequence used to overexpress miR-139-5p. The empty vector containing no insert was used as control vector. (B) Transduction efficiency was analyzed by GFP expression in the BM of reconstituted mice. (C) Expression of miR-139-5p was measured by quantitative RT-PCR in LSK and LK cells purified from reconstituted mice. Data represent the mean ± SEM (N = 3). (D) Flow cytometric analysis of hematopoietic progenitors with CD34 and FcγRIII expression (left) in LK population (Lin⁻c-Kit⁺Sca-1⁻). Average frequency of CMP (CD34^hiFcγRIII⁺), GMP (CD34⁺FcγRIII^hi), and MEP (CD34⁻FcγRIII⁻) in LK subsets is depicted (right). (E) Representative flow cytometric profiles of LSK (Lin⁻c-Kit⁺Sca-1⁺) (left) and average frequency of LSK subsets in reconstituted mice (right).