Skeletal muscle myosin promotes thrombin generation when 0.1 pM or 0.5 pM TF is added to pooled normal plasma both in the absence and the presence of 4 µM phospholipid vesicles. CTI (final, 50 µg/mL) was added to freshly thawed pooled normal human plasma (George King Bio Medical Inc., Overland Park, KS). Once-thawed pooled plasma (30 μL) was incubated with various indicated concentrations of myosin for 10 minutes at 37°C. Then, to initiate thrombin generation in the absence (A,C) or presence (B,D) of phospholipid vesicles (4 µM final) (PCPS, 80%/20% wt/wt), fluorogenic thrombin substrate solution (I-1140) with TF at either 0.1 pM (A,B) or 0.5 pM (C,D) (final concentration, as indicated) and CaCl₂ (11 mM final) was added.