Alloreactivity of post-dUCBT blood T cells toward third party HLA class II molecules. (A) PBMCs from 6 patients (patients 1, 4, 5, 8, 9, and 10) were propagated toward 1 to 7 mismatched alleles per patient (as specified in Figure 2A) and subsequently assayed toward a third party allele of the same family (as specified in the left column). The mean value of response of 1 to 4 observations per allele per patient is presented. The level of response was as follows: DR alleles, FI = 2.2 (median; range, 1.4-5.1; n = 6); DQ alleles, FI = 2.3 (median; range, 0.2-4.4; n = 4); DP alleles, FI = 1.5 (median; range, 0.2-2.8; n = 6). (B) PBMCs from 6 patients were propagated toward third party alleles (as specified in the left column) and assayed toward the same third party allele (Figure 2A). The level of response was as follows: DR alleles, FI = 3.3 (median; range, 1.3-21.2; n = 6); DQ alleles, FI = 3.9 (median; range, 1.4-8.8; n = 4); DP alleles, FI = 3.0 (median; range, 1.3-18.6; n = 6). The level of reactivity toward (empty) HeLa cell: median %CD137⁺/CD4⁺, 6.8 (range, 2.0-15.0; n = 11).