Phenotyping of MM-MØ from BM patient samples. (A) Multicolored staining of BM aspirates containing particles from active disease MM patients, CD38/CD138 (white), CD163 (red); FRβ, CD68, MIF, CD209, CD74, Clec5A, TNF, EGLN3 (green), as indicated. Upper panels represent panoramic views, whereas bottom panels are magnified ones. Nuclear 4′,6-diamidino-2-phenylindole (DAPI) appears in blue in all cases. (B) Plot showing the MFI for each marker in CD163⁺ TAM (n = 10 cases). Cells > 25 arbitrary units (a.u.) are considered positive, relative to negative control. Scale bars as indicated.