MIF receptors and signaling during MØ repolarization. (A) Flow cytometry analyses of intracellular and surface expression of MIF receptors CXCR4, CXCR7, CXCR2, and CD74 on M-MØ. (B) Expression levels of FOLR2, INHBA, and EGLN3, as determined by qRT-PCR on M-MØ treated for 24 hours with GM-CSF (1000 U/mL); 4-IPP (50 μM); AMD3100 (25 μg/mL); CCX733 (100 nM); SB225002 (300 nM) and α-CD74 blocking Ab (5 μg/mL); or GM-CSF in combination with all of them. Values in the absence of treatment are given an arbitrary value of 1. Result represents mean ± SEM of 4 independent donors. *P < .05; **P < .01; ***P < .001.