WDR1 is downregulated in activated MEG-01 cells. (A) WDR1 gene expression in MEG-01 cells was measured at baseline and after treatment with thrombin 1 U/L on collagen-coated coverslips at 6, 12, and 24 hours. (P = .04, 0.03, and 0.18, respectively, Student t test with Bonferroni correction). (B) Representative immunoblots probed for WDR1 in MEG-01 cells and quantification of the decrease in WDR1 protein after treatment with thrombin and collagen at 12 and 24 hours (P = .004 and <.0001, respectively, Student t test with Bonferroni correction). Activated MEG-01 cells were compared with control MEG-01 cells (absence of thrombin and noncoated coverslips). Data are mean ± SEM of 6 experiments. *P < .05, vs unstimulated (no thrombin or collagen) control MEG-01 cells. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.