WDR1 knockdown in MEG-01 cells affects cell size, adhesion, and spreading. MEG-01 cells were transfected with scrambled shRNA (control) or WDR1 shRNA (WDR1 KD). (A) WDR1 mRNA and protein expression in WDR1 KD and control MEG-01 cells. Data are mean ± SEM for 3 independent experiments. (B) WDR1 protein expression in WDR1 KD and control MEG-01, in the absence of agonist with noncoated glass coverslips or stimulated for 12 hours with thrombin 1 U/mL and adherent on collagen-coated coverslips and quantification. Data are mean ± SEM for 3 independent experiments (P = .004, .005, and .002 respectively, Student t test). (C) Adhesion of WDR1 KD and control MEG-01 cells (green) in the absence and presence of thrombin 1 U/mL on collagen-coated coverslips and adhesion quantification (P < .0001 and P = .0002, respectively, Student t test) Data are mean ± SEM for 3 independent experiments. (D) Assay for adhesion and spreading of WDR1 KD and control MEG-01 cells following stimulation with thrombin 1 U/mL and collagen 5 µg/mL using the xCELLigence system. The xCELLigence system measured impedance every 4 minutes for 24 hours, reported as a cell index value. Lines and bars = mean ± SEM of quadruplicates. Similar results were obtained in an additional 2 experiments. *P < .05 vs unstimulated control MEG-01 cells. AU, arbitrary unit.