WDR1 affects calcium influx. (A) Calcium concentration measured over time before and after addition of thrombin (0.1 U/mL, 1 U/mL, and 10 U/mL) at 180 seconds in MEG-01 cells. (B) Baseline calcium concentration was higher in WDR1 KD cells than controls (P < .0001, Student t test). (C) Quantification of calcium concentration change between baseline and peak calcium influx (left panel) (P = .0004 and P = .0001, respectively, Student t test); and between baseline and plateau levels, after 300 seconds (right panel). Data are median ± IQR of 10 experiments. *P < .05 vs control MEG-01 cells.