HIF-2 controls Epo transcription in NG2 cells. (A) Shown are individual Hct, RBC, and Hb values for Cre⁻ control (n = 10), NG2-Phd2^−/−Phd3^−/− (n = 8), NG2-Phd2^−/−Phd3^−/−Hif2a^+/− (n = 8), and NG2-Phd2^−/−Phd3^−/−Hif2a^−/− mice (n = 5). Bar graphs show serum EPO levels (sEPO) for Cre⁻ control (n = 4), NG2-Phd2^−/−Phd3^−/− (n = 6), NG2-Phd2^−/−Phd3^−/−Hif2a^+/− (n = 3), and NG2-Phd2^−/−Phd3^−/−Hif2a^−/− mice (n = 3). Data are represented as mean ± SEM; 1-way ANOVA followed by Tukey’s post hoc analysis; **P < .01 and ***P < .001 compared with Cre⁻ control group; ^fP < .05, ^ffP < .01, and ^fffP < .001 compared with NG2-Phd2^−/−Phd3^−/− group; ^&P < .05, NG2-Phd2^−/−Phd3^−/−Hif2a^+/− was compared with NG2-Phd2^−/−Phd3^−/−Hif2a^−/− group. (B) Striatal (ST), hypothalamic (HYT), cortical (CTX), and hippocampal (HP) Epo mRNA levels in Cre⁻ (n = 7), NG2-Phd2^−/−Phd3^−/− (n = 4), and NG2-Phd2^−/−Phd3^−/−Hif2a^−/− mice (n = 4). Data are represented as mean ± SEM; 1-way ANOVA followed by Tukey’s post hoc analysis; ***P < .001 compared with Cre⁻ control group and ^fffP < .001 compared with the NG2-Phd2^−/−Phd3^−/− group.