Figure 4
Figure 4. CSF1R deficiency affects fetal B-cell differentiation. (A) Total FL cells in E14.5 and E17.5 Csf1r+/+ (light gray) and Csf1r−/− littermate embryos (black) (n = 11-16 embryos per genotype). (B-C) Mean percentage (± SEM) of (B) ProB cells in E14.5 FL, and (C) ProB and PreB cells in E17.5 FL of Csf1r+/+ and Csf1r−/− littermate embryos (n = 11-16 embryos per genotype, 3-5 experiments). (D) (Left) Representative FACS profiles of total B (B220+CD19+) and mature B (B220+CD19+IgM+) cells in E17.5 fetal spleen of Csf1r+/+ and Csf1r−/− littermate embryos (4 experiments). Numbers represent mean percentage of gated populations relative to total spleen cells. (Right) Mean percentage (± SEM) of total B cells and mature B cells out of total E17.5 spleen cells in Csf1r+/+ and Csf1r−/− littermate embryos (n = 14-15 embryos per genotype). Statistical significance was tested between Wt (Csf1r+/+) and Csf1r−/−. ***P < .001; **P < .01; *P < .05. ns, not significant.

CSF1R deficiency affects fetal B-cell differentiation. (A) Total FL cells in E14.5 and E17.5 Csf1r+/+ (light gray) and Csf1r−/− littermate embryos (black) (n = 11-16 embryos per genotype). (B-C) Mean percentage (± SEM) of (B) ProB cells in E14.5 FL, and (C) ProB and PreB cells in E17.5 FL of Csf1r+/+ and Csf1r−/− littermate embryos (n = 11-16 embryos per genotype, 3-5 experiments). (D) (Left) Representative FACS profiles of total B (B220+CD19+) and mature B (B220+CD19+IgM+) cells in E17.5 fetal spleen of Csf1r+/+ and Csf1r−/− littermate embryos (4 experiments). Numbers represent mean percentage of gated populations relative to total spleen cells. (Right) Mean percentage (± SEM) of total B cells and mature B cells out of total E17.5 spleen cells in Csf1r+/+ and Csf1r−/− littermate embryos (n = 14-15 embryos per genotype). Statistical significance was tested between Wt (Csf1r+/+) and Csf1r−/−. ***P < .001; **P < .01; *P < .05. ns, not significant.

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