mMCP-11–deficient mice show ameliorated IgE-CAI response in the skin. WT and KO mice were sensitized with anti-TNP IgE and challenged with intradermal administration of TNP-OVA or control OVA in their ear skin to induce IgE-CAI. (A) Time course of ear swelling (Δear thickness: TNP-OVA − OVA) is shown (mean ± SD, n = 4 mice each). (B) Three days after the allergen challenge, mice were treated with intravenous injection of Evans blue dye, and 2 hours later their ears were subjected to photography followed by the measurement of leaked dye (mean ± SD, n = 3 or 4 ears each). (C-D) WT and KO mice were treated once a day during IgE-CAI with topical administration of SC560, meloxicam, or vehicle (ethanol) alone. In panel C, mice were subjected to the Evans blue dye leakage analysis as in panel B. Change in Evans blue dye leakage (TNP-OVA − OVA) is shown (mean ± SD, n = 3 or 4 mice each). In panel D, time course of ear swelling (Δear thickness: TNP-OVA − OVA) is shown (mean ± SD, n = 6 mice each). Data shown in panels A-D are representative of at least 3 independent experiments. *P < .05; **P < .01; ***P < .001.