Evolution of module enrichment in T cells from patients with GVHD. (A) Gene-set enrichment plot of selected NHP-WGCNA modules in patients who were diagnosed with GVHD at day 28 ± 7 posttransplant. Significant running enrichment scores were found in the dark-red and pink modules, but not for the orange and black modules for the day 28 samples. *FDR < 0.01. (B) Gene-set enrichment plot of selected NHP-WGCNA modules in patients who were diagnosed with GVHD day 60 ± 7 posttransplant. Significant running enrichment scores were found for both orange and black NHP gene modules at day 60 in patients with GVHD. Enrichment was also noted for the dark-red module, but not the pink module in the day 60 samples. *FDR < 0.01. (C) Heatmap showing the evolution of enrichment of the black module in patients diagnosed with GVHD at day 28 compared with day 60. Patients with GVHD were dichotomized into early GVHD (either at day 28 ± 7 posttransplant) or late GVHD (day 60 ± 7 posttransplant). Genes shown are those from the black NHP module exhibiting leading edge enrichment in patients at day 60 and their expression in either day 28 or day 60 GVHD patients relative to GVHD-negative controls.