Figure 4.
![Figure 4. HSF1A increased STAT3 function in AD-HIES patient EBV cells. EBV cells were treated with HSF1A (3 μM) for 48 hours, then stimulated with IL-21 (50 ng/mL). SOCS3 expression was measured by quantitative RT-PCR 120 minutes after stimulation (A). Total and phosphotyrosylated STAT3 and GAPDH were measured by Luminex 30 minutes after stimulation and results shown (B-D). STAT3 protein t1/2 determined (E-F; n ≥4 for each experiment). Comparisons of means marked by symbols *, **, #, ##, ^, ^^, ×, ××, ∞∞, ∨, ∨∨, +, &, &&, §, §§ indicate P < .01; ∞ indicates P < .05 ([A-D] analysis of variance [ANOVA]; [F] Student t test).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/128/26/10.1182_blood-2016-02-702373/5/blood702373f4.jpeg?Expires=1735500064&Signature=khEX-5HIdi9gkgf8kFYPArzS~fVeuCRI7dgnHk0l5ElI0FalCj06PSd84rwplSmwbiOTqGJULbstn-~7s3h6iCSxj~gX30l2xv9z382vV~ANHmM9To7DctqXJOnBoK4Klwi4hlRt9TWtIF3wx5X3H5ZM9ymBf5ie1nsBMQd-FH7BSXlanhRUmWDYnbk~fWFqN6HPBycxTFB0ub6iZPgjIpgf6b7XNzEYvOR~73fEx5d9~ywzdnYdf~raat1MHaBAOPKJ4GoP7lttAWsF9~VBvfv1K2Cq1KdhT0dBykmL4ZQb7PJLALih12~t2iIYWXYrC7UUKonBrk3Wk20pA-r-tQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HSF1A increased STAT3 function in AD-HIES patient EBV cells. EBV cells were treated with HSF1A (3 μM) for 48 hours, then stimulated with IL-21 (50 ng/mL). SOCS3 expression was measured by quantitative RT-PCR 120 minutes after stimulation (A). Total and phosphotyrosylated STAT3 and GAPDH were measured by Luminex 30 minutes after stimulation and results shown (B-D). STAT3 protein t1/2 determined (E-F; n ≥4 for each experiment). Comparisons of means marked by symbols *, **, #, ##, ^, ^^, ×, ××, ∞∞, ∨, ∨∨, +, &, &&, §, §§ indicate P < .01; ∞ indicates P < .05 ([A-D] analysis of variance [ANOVA]; [F] Student t test).
