GGA increased STAT3 function in AD-HIES patient EBV cells. EBV cells were treated with GGA (3 μM) for 48 hours, then stimulated with IL-21 (50 ng/mL). SOCS3 expression was measured by quantitative RT-PCR 120 minutes after stimulation (A). Total and phosphotyrosylated STAT3 and GAPDH were measured by Luminex 30 minutes after stimulation (B-D). STAT3 protein t_1/2 in a single representative experiment (E) and in multiple experiments (F; N = 3). Comparisons of means marked by symbols *, **, #, ^, ×,∨, +, &, §, indicate P < .01; ∞ indicates P < .05. Levels of HSP70 and HSP90 were determined without and with GGA in 2 experiments (G); *, # indicates P < .05.