In vivo regulation of FcγR profile on monocytes/macrophages by romiplostim in ITP mice. (A) Representative scattergrams of surface expression of CD11b⁺ splenic macrophages from an ITP mouse. R1 represented CD11b⁺ macrophages. (B) Representative histogram of mean fluorescence intensity (MFI) of FcγRI/CD64 and FcγRII/III/CD32/16 of splenic macrophages from 2 mice with romiplostim therapy and saline, respectively. (C) MFI of FcγRI/CD64 and FcγRII/III/CD32/16 on splenic macrophages with eltrombopag treatment or saline in ITP mice. (D) The mRNA levels of FcγRII/CD32 and FcγRIII/CD16 on splenic macrophages with eltrombopag treatment and saline in ITP mice. (E) Correlative analysis of the expressions of FcγRs and platelet counts. The differences between the 2 groups were determined by Mann-Whitney U tests. Correlative analysis was determined by nonparametric Spearman correlation.