FBXO11 deletion increases BCL6 protein levels in vivo. (A) Immunoblot analysis of FBXO11, BCL6, and actin in GC B cells isolated from spleens of FBXO11^+/+-Cγ1cre^tg/+ (WT), FBXO11^fl/+-Cγ1cre^tg/+ (HET), and FBXO11^fl/fl-Cγ1cre^tg/+ (KO) mice. Protein expression levels were quantitated by densitometric analysis, normalized to actin, and fold changes are displayed relatively to WT. (B) Average BCL6 mean fluorescence intensity measured by flow cytometry analysis and displayed as fold change relative to WT mice. n = number of analyzed mice, average ± SD; *P < .05 (1-way ANOVA).