Derepression of gluconeogenic genes and Epcam in Setdb1-deficient HSPCs. (A) Visualization of ChIP-sequence data of the levels of H3K9me3, H3K9/14ac, and H3K27me3 at the Fbp2, Fbp1, and Epcam gene loci in WT and Setdb1^Δ/Δ GMPs, using the Integrative Genomics Viewer. Schematic diagrams of these gene loci indicate their genomic structures. Exons and untranslated regions are demarcated by large and small black boxes, respectively. (B) DNA methylation status at the Fbp2 and Fbp1 gene loci detected by RRBS in WT and Setdb1^Δ/Δ ESCs and LSK cells and GMPs from WT and Setdb1^Δ/Δ mice. The proportions of methylated cytosine are depicted. (C) Manual ChIP assays for H3K9me3 and H3K9/14Ac at the Fbp2 loci using anti-H3K9me3 and H3K9/14Ac antibodies. The relative amounts of immunoprecipitated DNA are depicted as a percentage of input DNA. Data are shown as the mean ± SD (n = 3). (D) Flow cytometric profiles of BM Setdb1^Δ/Δ LSK cells and GMPs 2 weeks after the first injection of tamoxifen for Epcam expression.