ndrg1b overexpression can restore rag1 expression in zebrafish treated with the PTEN inhibitor SF1670. (A) Double WISH in 4.5-dpf embryos showing overlapping expression of indicated ndrgs (black) and rag1 (red). (B) rag1 expression in 4.5-dpf embryos in uninjected embryos (UI) treated with DMSO or 1 µM SF1670, and in SF1670-treated embryos overexpressing indicated ndrg mRNA at 200 pg per embryo. (C) Quantification of the thymus area in (B). Graph shows 3 independent experiments combined, n = 43 to 68 embryos per condition. ****P < .0001, 1-way analysis of variance (ANOVA).