ndrg1a and ndrg1b can rescue thymus size in fam49ab overexpressing embryos. (A-B) rag1 expression at 4.5 dpf is reduced in embryos injected with fam49ab mRNA. (C) rag1 expression is partially restored in embryos injected with fam49ab mRNA together with ndrg1b mRNA. (D-E) Coinjection of ndrg3a or ndrg3b together with fam49ab does not rescue rag1 expression. (F) Plotted thymus area measurements from 3 independent experiments combined, n = 57 to 73 embryos per condition. **P < .01; ****P < .0001, 1-way ANOVA. (G) Representative western blot of pools of embryos injected with 200 pg of indicated mRNA, lysed at 2 dpf, detecting pAkt and Akt. (H) Densitometric analysis of (G), 3 experiments combined. *P < .05, 1-way ANOVA.