Both models show a negative correlation between CD5 and CXCR4 but a positive correlation between CD5 and CD49d. (A) A representative figure showing matched circulating and migrated CD19⁺CD5⁺ CLL cells from a single patient harvested after 48 hours in the circulation system. Compared with each other, the migrated CLL cells are CD5^brightCXCR4^dim and the circulating cells CD5^dimCXCR4^bright. (B) A representative figure showing matched PB and LN CD19⁺CD5⁺ CLL cells from a single patient. The LN-CLL cells are CD5^brightCXCR4^dim and the PB cells CD5^dimCXCR4^bright. (C) For both the migrated and circulating CD19⁺CD5⁺ CLL cells, the MFI of the CD5 and CXCR4 were established and show a negative correlation. This was done on 8 patients and a color assigned to each patient. For each case, the dots surrounded by a colored box represents the result for the migrated and that surrounded by an oval in the same color represents the matched circulating result. In each case, the migrated cells have higher CD5 and lower CXCR4 than the circulating ones. (D) The same was done for the LN and PB CD19⁺CD5⁺ CLL cells from 11 patients. In each case, the LN-derived CLL cells have higher CD5 and lower CXCR4 (box) than those from the PB (oval). (E) In both the circulating/migrated model (Ei) and the LN/PB model (Eii), CD5 and CD49d positively correlate. Each solid circle represents the CD5 MFI plotted against the CD49 MFI for an individual patient sample.